izvkgtuИзвестия КГТУKSTU News1997-3071Калининградский государственный технический университет10.46845/1997-3071-2026-80-50-63izvkgtu-188Research ArticleБИОЛОГИЯ, ЭКОЛОГИЯ И РЫБНОЕ ХОЗЯЙСТВОBIOLOGY, ECOLOGY AND FISHERIESВлияние концентрации макроэлементов на рост, содержание фотосинтетических пигментов и состав жирных кислот микроводоросли Scenedesmus rubescensInfluence of macronutrient concentration on the growth, photosynthetic pigment content, and fatty acid composition of the microalga Scenedesmus rubescenshttps://orcid.org/0000-0002-3875-8437ЧижоваА. А.ChizhovaA. A.

Алена Алексеевна Чижова – аспирант 3-го года обучения Высшей школы живых систем

Калининград

Alena A. Chizhova – 3rd-year PhD student at the Higher School of Living Systems

Kaliningrad

alena_chizhova_2019@mail.ruhttps://orcid.org/0000-0003-4854-5459БуденковаЕ. А.BudenkovaE. A.

Екатерина Александровна Буденкова – кандидат технических наук, младший научный сотрудник Научно-образовательного центра «Промышленные биотехнологии»

Калининград

Ekaterina A. Budenkova – Candidate of Technical sciences, junior researcher at the Scientific and Educational Center "Industrial Biotechnology"

Kaliningrad

budenkovakaterina@gmail.comhttps://orcid.org/0009-0000-4477-2764ШушаринВ. С.ShusharinV. S.

Владимир Сергеевич Шушарин – лаборант Научно-образовательного центра «Промышленные биотехнологии»

Калининград

Vladimir S. Shusharin – Laboratory Assistant at the Scientific and Educational Center "Industrial Biotechnology"

Kaliningrad

vova-shusharin@mail.ruhttps://orcid.org/0000-0002-7333-8411ДышлюкЛ. С.DyshlyukL. S.

Любовь Сергеевна Дышлюк – доктор технических наук, доцент, заведующая лабораторией микробиологии и биотехнологий

Калининград

Lyubov S. Dyshlyuk – Doctor of Technical sciences, Associate Professor, Head of the Laboratory of Microbiology and Biotechnology

Kaliningrad

soldatovals1984@mail.ruБалтийский федеральный университет имени Иммануила КантаРоссияImmanuel Kant Baltic Federal UniversityRussian Federation2026310120260805063Copyright © Чижова А.А., Буденкова Е.А., Шушарин В.С., Дышлюк Л.С., 20262026Чижова А.А., Буденкова Е.А., Шушарин В.С., Дышлюк Л.С.Chizhova A.A., Budenkova E.A., Shusharin V.S., Dyshlyuk L.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.klgtu.ru/jour/article/view/188

Увеличение выбросов парниковых газов и истощение запасов ископаемого топлива ставят под угрозу устойчивое развитие и здоровье населения. В этих условиях возрастает интерес к микроводорослям, которые способны смягчить эти вызовы благодаря высокой продуктивности и широкому спектру применений. Виды рода Scenedesmus представляются перспективным сырьем для производства биодизеля и получения пигментов. В настоящей работе исследовалось влияние состава питательной среды на рост, накопление пигментов и жирнокислотный профиль микроводоросли Scenedesmus rubescens D-292. Культивирование штамма осуществлялось на среде Громова с различным содержанием KNO3 (0,1, 1,0 и 10 г/л), K2HPO4×3H2O (0 и 0,262 г/л), MgSO4×7H2O и NaHCO3 (0, 0,2 и 2,0 г/л). Максимальный рост штамма, накопление пигментов (10 мг/г сух. биомассы) и полиненасыщенных жирных кислот (ПНЖК) (65 % от общего количества жирных кислот (ЖК)) отмечены при избытке в среде источника серы (2,0 г/л MgSO4×7H2O) и дефиците азота, фосфора и углерода. Наибольший выход ЖК (229,9 мг/г сух. биомассы) зафиксирован при избытке в среде источника углерода (2,0 г/л NaHCO3) и ограничении других макроэлементов. В липидном профиле штамма преобладали пальмитиновая (14,4–17,5 %), олеиновая (13,8–29,1 %), линолевая (10,1–13,8 %) и α-линоленовая (19,7–36,7 %) кислоты. Несмотря на ограниченный потенциал штамма для производства биодизеля, он представляет интерес как источник натуральных пигментов и липидов, богатых ПНЖК и α-линоленовой кислотой (до 37 % от общего количества ЖК).

The rise in greenhouse gas emissions and the depletion of fossil fuel reserves threaten sustainable development and public health. In this context, microalgae are receiving increasing attention owing to their high productivity and broad biotechnological potential. Species of the genus Scenedesmus are considered promising raw materials for biodiesel production as well as for obtaining natural pigments with diverse biological activities. This study examines the effect of nutrient medium composition on the growth, pigment accumulation, and fatty acid profile of the microalga Scenedesmus rubescens D-292. The microalga has been cultivated in Gromov’s medium with varying concentrations of KNO3 (0.1, 1.0, and 10 g L-1), K2HPO4×3H2O (0 and 0.262 g L-1), MgSO4×7H2O и NaHCO3 (0, 0.2, and 2.0 g L-1). The highest growth rate, pigment content (10 mg g-1 dry biomass) and proportion of polyunsaturated fatty acids (65% of total fatty acids) has been observed under sulfur-rich conditions (2.0 g L-1 MgSO4×7H2O) combined with nitrogen, phosphorus, and carbon limitation. The highest fatty acid yield (229.9 mg g-1 dry biomass) has been obtained under carbon-rich conditions (2.0 g L-1 NaHCO3) with limited availability of other macronutrients. The lipid profile of the strain has been dominated by palmitic (14.4–17.5 %), oleic (13.8–29.1 %), linoleic (10.1–13.8 %), and α-linolenic (19.7–36.7 %) acids. Although the strain exhibits limited potential for biodiesel production, it demonstrates potential as a source of natural pigments and lipids rich in polyunsaturated fatty acids, particularly α-linolenic acid (up to 37% of total fatty acids).

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The authors declare that there are no conflicts of interest present.